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To prepare the plasma samples, 50 L of plasma was transferred to a 2.0 mL centrifuge tube, and 150 L of the precipitating agent (0.1% formic acid in acetonitrile) was added
To measure the reverse reaction rates, we mixed a pre-equilibrated mixture of RT (20 M) and GSH (10 or 5 mM) with an equal volume of PBS and followed the disappearance of RT-GSH fluorescence (Fig
BV has a cytotoxic impact on tumor cells at concentrations higher than 1 g/mL (Based on the cellular lineage)
Glutathione is a naturally produced antioxidant found in every cell of the body
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