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Briefly, the lentiviral vector containing inserts of interest and three packaging system plasmid (Rev,Gag and VSVG) were mixed and transfected into 293 T cells with EndoFectin-Lenti reagent (GeneCopoeia, USA), and then the virus containing culture medium was collected 48 h after transfection and spun for 10 min at 3000 r.p.m

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