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By binding to copper at the active site of tyrosinase and interfering with melanin synthesis intermediates, glutathione reduces overall melanin formation. As a powerful antioxidant, glutathione also reduces free radicalinduced oxidative stress, which is one of the triggers for increased tyrosinase activity and melanin production
Samples were then incubated at room temperature for 24 h with goat anti-chicken Alexa Fluor 488 (1:1000 dilution
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