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doi: 10.1007/s10157-021-02108-6 124 JaworskaKHucTSamborowskaEDobrowolskiLBielinskaKGawlakMet al
Although routine B12 supplementation in patients on metformin therapy is not the standard of care (279), it is reasonable to monitor vitamin B12 levels in patients on metformin, particularly for individuals on long-term metformin (102
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Immunoblotting Cells were washed with PBS and lysed with lysis buffer (150 mM NaCl, 20 mM EDTA, 50 mM Tris-HCl, 0.5% NP-40) containing protease and phosphatase inhibitors
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