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Total glutathione lysis reagent and GSSG lysis reagent, which contains alkylating agent N-ethylmaleimide (25 mM) were prepared following manufactures protocol and added to respective wells along with blank for background

Since ceramides are the major lipid components of the skin, they are crucial for maintaining skin barrier and hydration

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It can take many years (approx 20) to develop a deficiency disease in those altering their diet to one lacking in B12 due to reabsorbtion however, if failure to absorb leads to B12 deficiency disease occur within a few years (3-4)