In parallel, GLP-1 also prevents MG-induced beta cell apoptosis through PKA and PI3K-dependent pathway
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The membranes were blocked with 5% non-fat milk in TBST for 1 h at room temperature and then incubated overnight at 4 C with primary antibodies against ABCA2 (Proteintech, 20681-1-AP, 1:1000), SGPP1 (Biorbyt, orb389350, 1:800), CERS2 (Affinity, DF13124, 1:1000), and GAPDH (Cell Signaling Technology, #5174, 1:2000) as a loading control
Avoid doubling up on doses, and always return to your regular schedule afterward