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it depends on your research protocol and preferred dosing volume
Patients with higher initial body fat percentages generally lose a greater proportion of fat relative to lean tissue
After 30 minutes of incubation at 37 C in the dark, the percentage of PI positive cells was determined by using a flow cytometer (BD FACSCanto II, BD Biosciences), with 488 nm excitation laser and 585/42 nm band pass filter
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