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All cells were cultured in an endothelial cell-specific medium for human umbilical vein endothelial cells, which was supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (PCM-H-040, ZQXZbio, China)

For this, 10 l of HEK293T cells were seeded with 10,000 cells/well into white 384-well plates (Greiner, 784075) and the cells were allowed to attach for 24 h at 37 C and 5 % CO 2

In the Pro-Body catalogue, BPC-157 PEN 20 mg appears exclusively as a Research Use Only reagent with HPLC purity 98%, MS Q-TOF confirmation (sequence and salt) and a COA for every batch (concentration mg/ml, number of units)

In contrast to the French study, a recent study based on nationwide data from Korea used an active-comparator new user cohort design and propensity score weighting to control for confounding