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Blood Clotting Problems Glutathione injections may disrupt blood clotting, potentially raising the chances of bleeding and bruising 5

Given the potential of reduced gliotoxin to thiolate cellular proteins, we speculate that reduced gliotoxin may be sequestered into intracellular vesicles where it is converted to the oxidized form, by an unidentified activity, prior to release from the cell by an exocytotic mechanism complementary to GliA-mediated efflux (Figure 7)

To verify that the SAT1 disruption cassettes were successfully excised from GDH2/gdh2 and GDH3/gdh3 strains, genomic PCR was used with primers C1 and C4 to amplify the region between the upper end of 5 flank sequences to the lower end of 3 flank sequences to make sure that both alleles of each gene were removed (Supplementary Fig