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The buffer was exchanged on column from solubilization buffer to wash buffer comprised of 20 mM HEPES pH 7.5, 50 mM NaCl, 0.1% DDM, 1 mM MgCl 2 , 5 mM -ME, 10 M GDP, 0.2 g/ml leupeptin, and 150 g/ml benzamidine

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