J Invest Dermatol 125:685691 Swetter SM, chen FW, Kim DD, Egbert BM (2015) Imiquimod 5% cream as primary or adjuvant therapy for melanoma in situ, lentigo maligna type
(PubMed) Carr AC, Cook J
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Membranes were stripped for 20 min at room temperature using a Blot Restore Membrane Rejuvenation kit (Sigma-Aldrich, 2520-M), then washed twice with TBS-T, re-blocked with BSA 5% for 30 min and incubated with mouse-anti--actin (Sigma-Aldrich, A1978) 1:3,000 in BSA 2.5% for 4 h at room temperature and then incubated with a sheep-anti-mouse (Amersham, NA931) secondary antibody at 1:5,000 in BSA 2.5% for 2 h