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The dosing protocol directly affects the magnitude of IGF-1 elevation, which is the primary biomarker endpoint in much of the published GH-axis research
Animal Model and Ethics Statement Wistar rats from the breeding colony at the Universidade do Extremo Sul Catarinense (UNESC), Brazil, were housed four times per cage under controlled conditions of temperature (22 1 C), relative humidity (4555%) and light-dark cycle (12:12 p.m., light on at 6 a.m.)
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Plasma preparation We separated the resulting plasma into four preparation conditions that differed in anticoagulant used during sample collection, storage temperature, and time to deproteinization
