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At the indicated time (1, 2, 4, 6 and 22 h) an aliquot of 10 L was removed from each experimental reaction and pipetting into 100 L of 1% TFA solution to quench the reaction ( t = 0 min was determined using the reaction without enzyme)
Effect of rimonabant and metformin on glucose-dependent insulinotropic polypeptide and glucagon-like peptide-1 in obese women with polycystic ovary syndrome
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References 1 Wu, G., Fang, Y.-Z., Yang, S., Lupton, J
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