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Briefly, mostly ring-stage parasites were incubated at 0.2% initial parasitemia and 1% hematocrit with a range of CB-27 concentrations at 37C for 72 h in 96-well plates as previously described (Ekland et al., 2011)

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Under normal conditions, the gut microbiota secretes neurotransmitters (e.g., Gamma-Amino Butyric Acid - GABA, serotonin) and trophic factors (e.g., brain-derived neurotrophic factor - BDNF), which is related to neuronal communication, maintenance, and survival of the brain through neurotrophic support