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Notably, commonly used cell culture media (for example, DMEM, RPMI and F12) contain supraphysiological levels of cystine but lack GSH and cysteinylglycine

Plus, the symptoms of low B12 can be very subtle, or they may be confused for symptoms of other conditions

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coli strain XL1-Blue and purified by affinity chromatography using an elution buffer containing 200 mM imidazole, 300 mM NaCl and 50 mM sodium phosphate, pH 8.0 (refs 11, 12, 18, 33, 53)