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2.8 Immunoblot analysis Total protein was isolated from PIG1 and PIG3V cells disrupted in RIPA lysis buffer (Beyotime, China) with protease and phosphatase inhibitor cocktails, and quantified via BCA assay (Beyotime, China)
Growth factors and cell migration: The peptide boosts growth factor levels (such as fibroblast growth factor (FGF) and vascular endothelial growth factor (VEGF)) and increases cell adhesion proteins (integrins)
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