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The cell pellet was thawed, homogenized, and lysed in a buffer containing 20 mM HEPES pH 7.5, 50 mM NaCl, 2 mM MgCl 2 , and complete protease inhibitor cocktail tablet (Roche), and the membranes were collected by centrifugation at 40,000 g

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Current trials also exclude people with diabetes, recent heart problems, and eating disorders, which may indicate future contraindications once approved