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2.12 Immunofluorescence The aorta (5 microns thick) was subjected to antigen retrieval by heating it in 10 mM citrate buffer (pH 6.0) at 95 C for 10 min after decalcification and hydration
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Early identification facilitates timely intervention, improves outcomes, and reduces the risk of severe complications (Wroska et al., 2024)
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