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Recommend a personalized treatment plan that may combine multiple techniques for optimal results
Unless indicated otherwise, cystine-free medium was prepared by supplementing cystine-, methionine- and glutamine-free DMEM (Gibco, CA, USA) with 100 M methionine, 4 mM glutamine, 1 mM sodium pyruvate, 0.2% bovine serum albumin (BSA), 100 U/ml penicillin and 100 g/ml streptomycin
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Caloric restriction mimetics against age-associated disease: targets, mechanisms, and therapeutic potential
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