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To generate Gpx4 -/- and Acsl4 -/- clones by CRISPR Cas9 gene editing, transfection was performed for 48 h followed by puromycin (Gibco, A1113803) selection for 10 days and subsequent seeding onto 96-well plates with one cell per well for expansion
Ya que los cidos grasos al llegar al citoplasma, se unen a una molcula de coenzima A (acil-coA), que es impermeable a la membrana mitocondrial
Khawaji A, Jaly A, Bakri H, et al
Additionally, the same study reported a decrease in cigarette smoking among participants who were smokers, suggesting potential cross-substance benefits of GLP-1RAs in this population (Hendershot et al., 2025)
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