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After mincing to small pieces, they were treated with collagenase type V (Sigma-Aldrich, C9263) and trypsinEDTA (Gibco, 25200-056) and suspended in DMEM supplemented with 1% w/w penicillinstreptomycin and 10% FBS

Commonly used psychoactive agents are not sufficient as such to correct but commonly even worsen problems with circadian rhythm [82, 83]

Peptides may offer slower but more sustainable results by improving metabolic health and hormonal balance, while Ozempic provides faster weight loss through appetite suppression