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For analysis of the effect of cell culture conditions, hES-MSCs were seeded at densities of 5 10 3 and 2 10 3 cells/cm 2 , incubated for 19 h, stained with FreSHtracer, and the fluorescence ratio (F 510 /F 580 ) was measured as described above
The latter is better, but we recommend methylcobalamin, as it includes a methyl group and directly aids in lowering homocysteine levels and in fixing nerve damage, two issues often faced by pernicious anemia and B12 deficiency patients
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