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chronic studies) Common experimental uses include: ROS modulation studies Cytoprotection and viability assays Redox-sensitive signaling pathway analysis Biochemical & Enzymatic Assays: GSH is widely used as a substrate or cofactor in assays involving: Glutathione peroxidase (GPx) activity Glutathione S-transferase (GST) function Redox cycling and antioxidant capacity measurements Concentrations are adjusted based on assay sensitivity and enzyme kinetics