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In vitro bioactivity assays CHO-hIR cells were cultured in DMEM complete medium, consisting of fetal bovine serum (50.6 mL), sodium pyruvate (5.6 mL), non-essential amino acid solution (5.6 mL), and methotrexate (MTX) at a concentration of 4 mg/mL (0.56 mL added to 500 mL of DMEM basal medium)
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The study included certain demographics and health conditions, so the results might not apply equally to everyone
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