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First, for sample normalization, we employed Quantile normalization, which adjusts the data to conform to a common distribution 81 , thereby minimizing technical variability

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Clinical trial data from the SURMOUNT series shows these incidence rates: Nausea: approximately 20 percent of patients Diarrhea: approximately 16 percent of patients Vomiting: approximately 9 percent of patients Constipation: varies by dose level Decreased appetite: expected therapeutic effect Abdominal pain: less common but reported Most gastrointestinal side effects are mild to moderate and occur during the first few weeks of treatment or after dose increases