Four fields of view per culture well were imaged, and parasites and host nuclei were counted using its Gen5 software
In order to test whether this is sufficient to induce oxidative stress in parasites, we measured thiol species following treatment with 30 M of SFN for 3 h, compared to untreated control
In addition, the immunomodulatory potentiality of these compounds to trigger T helper type 1 (Th1) cells and cytokine-mediated immune response for the substantial induction of proinflammatory cytokines, thus, Se, selenoproteins, and parasite selenoproteins could be further investigated to find possible vaccine antigens
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