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Instead of tying a knot, tuck 1 end of the strap beneath the wrapped section of the tourniquet

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In avascular tissue zones, HIF-1 is stabilised (it is normally rapidly degraded under normoxic conditions by prolyl hydroxylase enzymes) and drives the transcription of dozens of hypoxia-response genes, including VEGF, erythropoietin, glucose transporters, and several matrix metalloproteinases involved in remodelling

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