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Briefly, the construct was produced by stable expression and secretion from CHO-K1 cells and then purified by a sequential combination of 9E10 antibody-affinity chromatography and size-exclusion chromatography as previously described 35 , with the final sample being prepared at a concentration of 0.2 mg mL 1 in a 24.8 mM Tris-HCl (pH 8.0), 137 mM NaCl, 2.7 mM KCl plus 0.02% NaN 3 (TBSA)

This lack of dose standardization complicates direct comparisons and may contribute to the discrepancies in reported outcomes regarding physical development