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The activity was followed by measuring the quantity of radioactive diol formed in the aqueous phase using a scintillation counter (TriCarb 2810 TR, Perkin Elmer, Shelton, CT)

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Research has revealed a unique protease activation phenomenon in MELAS: LONP1 exhibits subcellular compartmentalized aggregation in primary cells from patients with MELAS, with its protein abundance particularly elevated in cell populations enriched with mutant mtDNA

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