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Following the injection, imaging was performed using an IVIS Spectrum system (PerkinElmer) at various time points: 1 h, 4 h, 7 h, 12 h, 24 h, 36 h, 48 h, and 60 h post-injection, with an excitation wavelength of 780 nm and a fluorescence emission wavelength of 840 nm
From this perspective, it seems that Lipinskis Rule of Five, which was developed based on the solubility and permeability data of small orally administered molecules, might not accurately predict oral bioavailability for peptides
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