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After the reaction, the absorbance of resorufin at 570 nm was measured, and for each sample the percentage of resorufin inhibition rate was calculated and converted to activity of SOD using the formula: (inhibition rate 50% = 1 U SOD activity)
Many cardiovascular risk factors such as diabetes mellitus, hypertension and hyperlipidemia are demonstrated to stimulate the interaction of Cav-1 and eNOS, reduce nitric oxide production resulting in endothelial dysfunction and subsequently the formation of atherosclerotic lesion 41,42,43
This restoration of metabolic cofactor availability enables enhanced function of NAD+-dependent enzymes including sirtuins, poly(ADP-ribose) polymerases, and various deacetylases that regulate cellular metabolism, stress responses, and aging processes
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