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It is synthesised in the cytosol by glutamate-cysteine ligase (EC 6.3.2.2) and glutathione synthetase, with the first, rate-limiting step feedback-inhibited by glutathione itself and intracellular concentrations typically 1 to 8 mM (Griffith, Free Radical Biology and Medicine, 1999)

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In comparison to the placebo group, T2DM subjects post-3 months supplementation with L-GSH had a significant several-fold increase in the production of both IFN- ( Figures 6B, C ) and TNF- ( Figures 6D, E ) in the granuloma supernatants from the L-GSH group at both 8-days and 15- days post in vitro BCG infection