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For drug treatments and qPCR analyses, hiPSC-CM were seeded on gelatin- or Matrigel-coated 12-well plates at 500,000 cells/well in medium containing RPMI 1640, B-27 supplement, 10 M ROCK inhibitor and 10% fetal bovine serum

However, the previous studies could not avoid the confounding effects of the endogenous carnitine background when attempting to evaluate the effects of administering exogenous l- or d-carnitine

Retatrutide is the most studied of the triple agonists, with the strongest public efficacy data, which is why it dominates the conversation