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qPCR analysis qPCR for selected wheat genes was conducted with the iQ SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA) on a BioRad-iCycler detection system as described previously (Liu et al., 2010)

Bibcode:2015PLoSO..1019479L

The expression of nutrient transporters, such as GLUT1 and LAT1, has been shown to be high in both, the BBB and in glioma (207, 208), which led to the development of different transporter-utilizing anti-cancer drug delivery strategies in order to reach the cancer cells residing beyond the intact BBB (Table II)

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