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The sections were developed with chromogenic agent A, B, and C using DAB kit (Sigma-Aldrich) for 6 min, stained with hematoxylin for 30 s, dehydrated in ascending series of alcohol (70%, 80%, 90%, and 95% ethanol and anhydrous in sequence) for 2 min, immersed twice in xylene for 5 min and sealed with neutral resin before observation and counting under an upright microscope (BX63, Olympus Optical Co., Ltd., Tokyo, Japan)
According to the bias assessment using the Cochrane Collaborations risk of bias tool, this study had a lower risk of bias
Composite (Comp) score, primary objective The Comp endpoint is comprised of three critical factors that characterize sarcopenia, i) muscle mass loss, ii) muscle strength loss, and iii) physical activity
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