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(2015) investigated the gut-brain-kidney axis and revealed that CKD increases gut permeability, leading to the translocation of bacteria and their products, such as LPS, into the bloodstream

The remaining fat particles were digested with 0.2% collagenase (Gibco, Billings, MT, USA) in a water bath shaker at 37 C for 30 min, and the mixture was centrifuged at 1000 rpm for 5 min to remove undigested fat

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