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Parker TD, Cash DM, Lane CAS, Lu K, Malone IB, Nicholas JM, et al

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Then, GPR43 (50 nM), GPR109a (50 nM), and NC siRNA (50 nM) were delivered into the HepG2 cells (~40% confluent density) and cultured in Opti-MEM (Gibco) for 6 h, after which the transfection mixture was replaced with normal medium