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Lipids were separated at a flow rate of 0.3 ml min 1 (column temperature 50 C) using the following gradient: 010 min, 30% to 80% B (curve 5)

Pramlintide, the first generation (1990s) In the 1990s, the pharmaceutical company Amylin Pharmaceuticals developed Pramlintide , a modified amylin with three proline substitutions at positions 25, 28 and 29