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pJB-GST-HTS and the PCR fragment were prepared by BamHI and EcoRI digestion (Fw: AAAGGATCCATCATCATCATCATCATGGTCCGCTGGGCGTTCGTGGTATGGCTAGCAAAGGAGAAGAACTC, Rev: AAAGAATTCTCAGTTGTACAGTTCATCCATGCCATG)

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This is short-term stress, the fight-or-flight response, mediated by the hormones epinephrine and norepinephrine from the adrenal medulla via the SAM pathway

These conditions include aging and age-associated chronic muscle conditions such as cachexia 248 , sarcopenia 124,249,250 , inclusion body myositis (IBM) 114,251,252 , and neuromuscular diseases like Duchennes muscular dystrophy (DMD) 253,254