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pSSBR was then treated with 10 units of Nb.BtsI (NEB) at 37 C for 4 h, generating a plasmid with single-strand breaks (nicks) on opposing strands, separated by a distance of 36 bp

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With the GSH-ee experiments, mice were injected intraperitoneally with either vehicle (saline) or Glutathione ethyl ester (Cayman Chemical, 14953) for 4 days ((17-20) days post AAV-TBG-Cre treatment)

4), a patient exhibiting clinical symptoms of cholestatic disease with no known cause should undergo genetic testing to look for mutations in the known genes that cause cholestasis [19]