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The ERK-specific inhibitor (U0126) was added 30 min before the addition of liraglutide
A similar phenomenon has been observed at the calcitonin receptor, another class B1 GPCR, where human calcitonin, with a fast off-rate, turns over G protein faster than salmon calcitonin, which has a slow off-rate, and this was related to differences in the residency time of the G protein on the receptor and to the sensitivity of the G protein to GTP 35
These molecules are small enough to pass easily through cell membranes and work inside cells
Bailey, J
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