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5 Specific enzymes in intracellular lysosomes or tumor microenvironments can selectively break the linker, for example, proteases in lysosomes hydrolyzing peptide bonds contained in dipeptide or tetrapeptide linkers, glycosidases hydrolyzing -glucuronides and -galactosides, phosphatases hydrolyzing phosphoesters and pyrophosphates, and sulfatases hydrolyzing sulfates

Cultures were subsequently cooled in an ice water bath for 15 min and centrifuged (4.000 g , 15 min, 4 C)

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