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doi: 10.1038/s41569-022-00760-3 73 TrujilloJMNufferWSmithBA
The tissue sections of plerocercoids and adult worms was first retrieved after microwaving for 20 min with a 0.01 M citric acid buffer (pH 6.0), blocking with 5% normal goat serum in PBS, then incubating at 37C or 1 h with a 1: 10 dilution of anti-rSmGST serum, serum of mice infected with plerocercoids, and normal mouse serum or PBS, respectively
Additionally, polypharmacy, defined as the concurrent use of five or more medication classes [28], is more likely to occur in older adults and is associated with severe hypoglycemia
Nakamichi et al
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