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If you used sterile water (without a preservative), its lifespan is drastically shorterwe're talking 24 hours at most before the risk of bacterial growth becomes unacceptable
Chromatographic conditions employ a C18 reversed-phase column, with acetonitrilewater solution (adjusted to an appropriate acidity with phosphoric acid) as the mobile phase
After centrifugation at 1000 x g for 10 min, the supernatant (S1) was centrifuged for 20 min at 17.500 x g to yield the supernatant S2 and the pellet P2 (crude synaptosomal fraction)
PMC Article PMC12446177
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