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Potentially the lung may be utilizing GSH from the plasma through an intact uptake mechanism shown to occur in the lung [27], which is then apically transported into the ELF, resulting in no net change in lung tissue GSH but a drastic increase in ELF GSH

For the quantification of these metabolites in TIF samples, 5 l samples were extracted in 45 l ice-cold extraction solvent (75% acetonitrile, 25% methanol and 0.1% formic acid) containing isotope-labelled internal standards (36 M 13 C 2 , 15 N-GSH, 0.92 M 13 C 4 , 15 N 2 -GSSG and 10 M D 4 -cystine)

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