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In vivo study Animals and experimental design All animal procedures were approved by the Ethics Committee of the Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Egypt (Approval No.: PSAS-BSU-HAREC.025/08/08), and conducted in accordance with institutional, national, and international animal care guidelines

Melanin pigmentation of the oral cavity is sufficiently common among members of all dark-skinned races, regardless of nationality, to be considered physiologic

Kidney Function: Regular blood tests (creatinine, eGFR) for Tirzepatide users, as impaired kidney function can affect metabolism

Then the means of these values were calculated for each study group