Metabolomics analysis and data processing Metabolite extraction was performed by adding a cold solvent mixture of acetonitrile: isopropanol: water (3:3:2, v/v/v) to a homogenized tissue aliquot of 25 mg sea urchin gonad followed by centrifugation and drying of the supernatant as per Fiehn 37
Another possibility is that the physical side effects of Ozempic, which can include nausea, vomiting, diarrhea, and abdominal pain, may contribute to feelings of discomfort or distress, potentially leading to depressive symptoms
**Carnipure is used under license
Liu, B., Zhang, M., Zhao, J., Zheng, M