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In vitro studies demonstrated that hesperetin, the aglycone of hesperidin, stimulated the phosphorylation of key proteins (Src, Akt, AMP kinase (AMPK), and eNOS) in bovine aortic endothelial cells, leading to enhanced NO production

Maintaining a consistent rotation schedule across different injection sites, including the outer thighs, upper abdomen, and flanks, allows each site adequate time to recover between injections

2.5.4 Skin apparent evaluation After establishing the UV-induced skin photoaging model, the dorsal skin of the mice displayed characteristic signs, such as localized thickening, laxity, prominent deep wrinkles, and hyperpigmentation, confirming successful model construction