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Mice were anesthetized with isoflurane and injected with 0.5 l of GRAB-DA2m virus unilaterally in the nucleus accumbens (stereotaxic coordinates A/P 1.2, M/L 0.6, D/V 4.5 mm from bregma)
Molecular biologists studying GHS-R1a receptor signaling, biochemists mapping downstream cAMP or calcium flux responses, and cellular researchers examining proliferation or differentiation pathways all find utility in this compound
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To account for potential dropout of up to 20%, a total of 24 subjects were planned for active treatment, and 12 subjects were planned for treatment with placebo (total for both placebo doses), leading to a total number of 36 subjects planned to start in the trial
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