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The libraries were quantified using KAPA Library Quantification kits for Illumina Sequencing platforms according to the qPCR Quantification Protocol Guide (KAPA BIOSYSTEMS, #KK4854) and qualified using TapeStation D1000 ScreenTape (Agilent Technologies, # 50675582)
Option 1: Discard and mix fresh Cost: Price of new vial Benefit: Guaranteed full potency Risk: None (besides cost) Option 2: Continue using Cost: None (already paid for) Benefit: Don't waste remaining peptide Risk: 10-15% potency loss means 10-15% less effectiveness Calculation: If vial costs $50 and you're at day 35 with half remaining, you're deciding whether to waste $25 to guarantee full potency, or accept 10-15% reduced effectiveness
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Riede, L
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